comparative proteomics Essay - 944 Words

Comparative Proteomics: Protein Profiler Lab by Jonathan Thulson Biology 113 October 6, 2013 Lab Partner: Vernon Morris INTRODUCTION Proteomics is the study of proteins. Their functions, interactions with other proteins, cellular locations and levels at which they are expressed. The purpose of this lab was to compare the proteins present in different species of fish to be able to determine which species of fish have the closest relation. This can be determined based on which two fish species have the most proteins in common with one another. The Central Dogma of biology is a process in which a gene made of DNA is transcribed by a messenger RNA and then translated into a protein. Based on the Central Dogma of†¦show more content†¦The proteins are also added to a Laemmli sample buffer in order to give each protein a negative charge so it is able to get pulled through the polyacrylamide gel. The next step is to put the gel into the electrophoresis module and to run it. It is run until the proteins have almost reached the bottom of the gel. A blue tracking dye is added to the Laemmli sa mple buffer in order to track the distance in which the proteins travel through the gel. If it is run for too long, the proteins will run off the bottom of the gel and it will mess up your results. Once the protein reach the bottom of the gel, the gel is stained in order to be able to see the individual bands of the different proteins. When the gel is stained, the protein distances will be able to be measured and compared. For a detailed procedure, refer to the Comparative Proteomics Kit I: Protein Profiler Module Lab Manual. RESULTS I did not get conclusive data from the gel I made. As you can see in figure 1, the bands that showed up on the gel were too cluttered to be able to measure them. So I could not compare protein bands between the fish species based on our gel. Instead, I used a default gel picture that another group did in the class to get my data. From their gel I was able to compare the different species. Table 1 shows the number of bands that were similar between the different fish species when they were compared. I was able to determine that fish species C (Tuna)Show MoreRelatedDirect Analytical Sample Quality Assessment (DASQ) for Biomarker Investigation591 Words   |  2 PagesThere are several important factors to increase the chances that a proteomic study based on CSF biomarkers becomes successful. For proteomics analysis, the standardisation of CSF collection protocols is a mandatory step to search and identify new biomarkers indicators of a disease status. This point represents a mil estone especially when samples are collected and stored in different centres. 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